This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0006431; Intron-spanning
This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate and mutations in this gene are thought to cause dicarboxylicamino aciduria also known as glutamate-aspartate transport defect. [provided by RefSeq Mar 2010]