This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0006122; Intron-spanning
The protein encoded by this gene functions to inhibit WNT proteins which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene and has been found to be epigenetically silenced in various cancers. [provided by RefSeq Jun 2010]