This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0057290; exonic
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus in which DNA polymerase alpha and primase are two key enzymatic components. Primase which is a heterodimer of a small subunit and a large subunit synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small 49 kDa primase subunit. [provided by RefSeq Jul 2008]