This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0057214; exonic
This gene encodes a member of the WD-40 protein family. The protein is proposed to function as a molecular scaffold for various multimeric protein complexes. The protein associates with several components of the extracellular signal-regulated kinase (ERK) pathway and promotes ERK activity in response to serum or other signals. The protein also interacts with egl nine homolog 3 (EGLN3 also known as PHD3) and regulates expression of hypoxia-inducible factor 1 and has been purified as part of the spliceosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]