This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0057014; exonic
Riboflavin kinase (RFK; EC 220.127.116.11) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al. 2003 [PubMed 12623014]).[supplied by OMIM Nov 2009]