This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0056671; exonic
The calpains calcium-activated neutral proteases are nonlysosomal intracellular cysteine proteases. The mammalian calpains include ubiquitous stomach-specific and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large catalytic subunits associated with a common small regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Nov 2010]