PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DFFB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046647; exonic

List Price:    $130.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5011068
Chromosome Location:   1:3775304-3775396question
Amplicon Length:   63

Gene Information

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DFFB
Gene Name:   DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Aliases:   CAD, CPAN, DFF-40, DFF2, DFF40
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000169598
Entrez:   1677
UniGene:   Hs.133089
Chromosome Mapping:   1p36.3

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