This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0045666; exonic
RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g. GTF3C1 MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs including 5S RNA (MIM 180420) tRNA and adenovirus-associated (VA) RNA of both cellular and viral origin.[supplied by OMIM Mar 2008]