This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0045268; exonic
MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 22.214.171.124). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al. 2004 [PubMed 14722266]; Thibodeau et al. 2008 [PubMed 18389477]; De Gassart et al. 2008 [PubMed 18305173]).[supplied by OMIM Mar 2010]