PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IDH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044840; exonic

List Price:    $130.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5009449
Chromosome Location:   2:209113368-209116269question
Amplicon Length:   103

Gene Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases which localize to the mitochondrial matrix and two NADP(+)-dependent isocitrate dehydrogenases one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions such as the conversion of 2 4-dienoyl-CoAs to 3-enoyl-CoAs as well as in peroxisomal reactions that consume 2-oxoglutarate namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq Jul 2008]

Gene Symbol:   IDH1
Gene Name:   isocitrate dehydrogenase 1 (NADP+), soluble
Aliases:   IDCD, IDH, IDP, IDPC, PICD
RefSeq:   NC_000002.11 NG_023319.1 NT_005403.17
Ensembl:   ENSG00000138413
Entrez:   3417
UniGene:   Hs.593422
Chromosome Mapping:   2q33.3

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