This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0044587; exonic
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin as well as alpha-1 protease inhibitor a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia and in the renal tubular response to proteinuria. This protein which is a member of the peptidase C1 family is a dimer composed of disulfide-linked heavy and light chains both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Apr 2012]