This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0044410; exonic
This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present thus coupling pre- and post-mRNA splicing events. Previously it was thought that two genes encode this protein RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein and this gene also uses multiple polyadenylation sites. [provided by RefSeq Jul 2008]