This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0044206; exonic
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor known as kinase insert domain receptor is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation survival migration tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors including Rab GTPase P2Y purine nucleotide receptor integrin alphaVbeta3 T-cell protein tyrosine phosphatase etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq May 2009]