This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0044091; exonic
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt 1996 [PubMed 8921381]).[supplied by OMIM Mar 2008]