This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0043883; exonic
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain a caspase recruitment domain (CARD) and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein releasing its mature activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]