This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0043431; exonic
This gene encodes one of the three alpha chains of type IX collagen which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules a heterotrimeric molecule and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans with multiple epiphyseal dysplasia 6 a form of chondrodysplasia and with Stickler syndrome a disease characterized by ophthalmic orofacial articular and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]