This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0043154; exonic
This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain consisting of a POU-specific domain and a homeodomain separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers in a sequence-specific manner. The POU family members are transcriptional regulators many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq Oct 2009]