This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0042246; exonic
DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein and it has a homolog on the X chromosome. The gene mutation causes male infertility Sertoli cell-only syndrome or severe hypospermatogenesis suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]