This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0042104; exonic
The protein encoded by this gene contains ARF-GAP RHO-GAP ankyrin repeat RAS-associating and pleckstrin homology domains. The protein is a phosphatidylinositol (345)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. [provided by RefSeq Sep 2008]