This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0042064; exonic
This nuclear gene encodes a mitochondrial matrix enzyme. Missense nonsense and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy it may play a role in that disease also. [provided by RefSeq Jul 2008]