This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0042034; exonic
This gene encodes a member of the intermediate filament family. Intermediate filamentents along with microtubules and actin microfilaments make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape integrity of the cytoplasm and stabilizing cytoskeletal interactions. It is also involved in the immune response and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment migration and cell signaling. Mutations in this gene causes a dominant pulverulent cataract.[provided by RefSeq Jun 2009]