This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0041870; exonic
The protein encoded by this gene is one subunit of a trimeric complex forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit suggesting a role as the regulatory subunit. In addition there is evidence of post-transcriptional regulation in this gene product either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain with clear tissue-specific preferences for the two isoforms. [provided by RefSeq Jul 2008]