This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0038601; exonic
This gene encodes the smallest subunit of dynactin a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions including ER-to-Golgi transport the centripetal movement of lysosomes and endosomes spindle formation cytokinesis chromosome movement nuclear positioning and axonogenesis. This subunit like most other dynactin subunits exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq Jul 2008]