This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0037983; exonic
This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase or Complex I a multimeric enzyme of the respiratory chain responsible for NADH oxidation ubiquinone reduction and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and as such is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq Mar 2010]