PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DIO1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037912; exonic

List Price:    $130.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5006316
Chromosome Location:   1:54370339-54370445question
Amplicon Length:   77

Gene Information

The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some but not all isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq Jul 2008]

Gene Symbol:   DIO1
Gene Name:   deiodinase, iodothyronine, type I
Aliases:   5DI, MGC130050, MGC130051, TXDI1
RefSeq:   NC_000001.10 NG_023306.1 NT_032977.9
Ensembl:   ENSG00000211452
Entrez:   1733
UniGene:   Hs.251415
Chromosome Mapping:   1p33-p32

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