This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0037779; exonic
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde a potent irritant and sensitizing agent that causes lacrymation rhinitis pharyngitis and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq Oct 2008]