PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SHH, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037736; exonic

List Price:    $130.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5006220
Chromosome Location:   7:155599083-155604529question
Amplicon Length:   151

Gene Information

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube the anterior-posterior limb axis and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE) a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome which is characterized by vertebral defects anal atresia tracheoesophageal fistula with esophageal atresia radial and renal dysplasia cardiac anomalies and limb abnormalities. Additionally mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq Jul 2008]

Gene Symbol:   SHH
Gene Name:   sonic hedgehog
Aliases:   HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
RefSeq:   NC_000007.13 NG_007504.1 NT_007741.14
Ensembl:   ENSG00000164690
Entrez:   6469
UniGene:   Hs.164537
Chromosome Mapping:   7q36

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