This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0037658; exonic
Alkylglycerol monooxygenase (AGMO; EC 22.214.171.124) cleaves the O-alkyl bonds of ether lipids which are essential components of brain membranes and function in cell signaling and other critical biologic processes. The reaction catalyzed by AGMO is dependent on tetrahydrobiopterin a metabolite structurally related to the vitamins folic acid and riboflavin (summary by Watschinger et al. 2010 [PubMed 20643956]).[supplied by OMIM Feb 2011]