This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0036786; exonic
Multisubunit vacuolar-type proton pumps or H(+)-ATPases acidify various intracellular compartments such as vacuoles clathrin-coated and synaptic vesicles endosomes lysosomes and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells where they play roles in urinary acidification bone resorption and sperm maturation. Multiple subunits form H(+)-ATPases with proteins of the V1 class hydrolyzing ATP for energy to transport H+ and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit an essential proton pump component (Blake-Palmer et al. 2007 [PubMed 17350184]).[supplied by OMIM Mar 2008]