This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0036599; exonic
This gene encodes the perlecan protein which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin prolargin collagen type IV FGFBP1 FBLN2 FGF7 and Transthyretin etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g. FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1 Silverman-Handmaker type of dyssegmental dysplasia and Tardive dyskinesia.[provided by RefSeq Mar 2010]