This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0034520; Exonic
This gene is a member of the protocadherin gamma gene cluster one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes subfamily B contains 7 genes and 2 pseudogenes and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large variable region exons are followed by a constant region containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq Jul 2008]