This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0033940; Exonic
Sialic acid-binding immunoglobulin (Ig)-like lectins or SIGLECs (e.g. CD33 (MIM 159590)) are a family of type 1 transmembrane proteins each having a unique expression pattern mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif or ITIM (see MIM 604964) and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al. 2000 [PubMed 11095983]).[supplied by OMIM May 2010]