This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0023686; Exonic
This gene encodes an epidermal growth factor-related protein that contains a cripto FRL-1 and cryptic domain. The encoded protein is an extracellular membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2 3 6 8 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq Mar 2010]