PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HBA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0020775; Exonic

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5004844
Chromosome Location:   16:227405-88713166question
Amplicon Length:   90

Gene Information

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2 which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   HBA1
Gene Name:   hemoglobin, alpha 1
Aliases:   CD31, MGC126895, MGC126897
RefSeq:   NC_000016.9 NG_000006.1 NT_010393.16
Ensembl:   ENSG00000206172
Entrez:   3039
UniGene:   Hs.449630
Chromosome Mapping:   16p13.3

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