This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0020644; Exonic
Degradation of glycine is brought about by the glycine cleavage system which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase) H protein (a lipoic acid-containing protein) T protein (a tetrahydrofolate-requiring enzyme) and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants one protein-coding and the other probably not protein-codinghave been found for this gene. Also several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq Jan 2010]