This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0019601; Exonic
This gene is intronless arose via retrotransposition of the phosphoglycerate kinase 1 gene and is expressed specifically in the testis. Initially assumed to be a pseudogene the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 13-bisphosphoglycerate to 3-phosphoglycerate during the Embden-Meyerhof-Parnas pathway of glycolysis in the later stages of spermatogenesis.[provided by RefSeq May 2010]