This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0019582; Exonic
This gene encodes the transcriptionally active subunit of the SIII (or elongin) transcription elongation factor complex which also includes two regulatory subunits elongins B and C. This complex acts to increase the rate of RNA chain elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites along the DNA template. Whereas a related protein with similar function elongin A is ubiquitously expressed the encoded protein is specifically expressed in the testis suggesting it may have a role in spermatogenesis. [provided by RefSeq Jul 2008]