This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0003487; Exonic
This gene encodes a member of the transient receptor potential (TRP) protein family which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction and has characteristics of a calcium-activated non-selective cation channel that carries Na+ K+ and Cs+ ions equally well but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+) and inhibited by higher concentrations. It is also a highly temperature-sensitive heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5 and has been shown to be imprinted with exclusive expression from the paternal allele. [provided by RefSeq Oct 2010]