This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0003167; Exonic
The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon suggesting a function of this protein in downregulating interferon responses independent of its isopeptidase activity towards ISG15. [provided by RefSeq Sep 2011]