This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0002640; Exonic
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene along with GTP catalyzes the formation of phosphoenolpyruvate from oxaloacetate with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin glucocorticoids glucagon cAMP and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq Jul 2008]