PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NDUFA5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002394; Exonic

List Price:    $130.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5001111
Chromosome Location:   7:123181281-123181457question
Amplicon Length:   147

Gene Information

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing water soluble "iron-sulfur protein" (IP) fraction of complex I although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq Jul 2008]

Gene Symbol:   NDUFA5
Gene Name:   NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
Aliases:   B13, CI-13KD-B, CI-13kB, DKFZp781K1356, FLJ12147, NUFM, UQOR13
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000128609
Entrez:   4698
UniGene:   Hs.651219
Chromosome Mapping:   7q32

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