This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0002140; Exonic
This gene encodes beta-tropomyosin a member of the actin filament binding protein family and mainly expressed in slow type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins and cause cap disease nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2009]