This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCED0001809; Exonic
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome esophageal squamous cell carcinoma trichoepitheliomas transitional cell carcinomas of the bladder as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq Jul 2008]