This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039991; exonic
This gene is contained entirely within intron 22 of the factor VIII gene; spans less than 2 kb and is transcribed in the direction opposite of factor VIII. A portion of intron 22 (int22h) containing F8A is repeated twice extragenically closer to the Xq telomere. Although its function is unknown the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq Jul 2008]