PrimePCR™ ddPCR™ Expression Probe Assay: TTN, Human


ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0032710; Intron-spanning

Fluorophore:   FAM
List Price:    $201.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5057108
Chromosome Location:   2:179665307-179666964question
Amplicon Length:   173

Gene Information

This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions a N-terminal I-band and a C-terminal A-band. The I-band which is the elastic part of the molecule contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline glutamate valine and lysine. The A-band which is thought to act as a protein-ruler contains a mixture of immunoglobulin and fibronectin repeats and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and therefore to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9 and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq Feb 2012]

Gene Symbol:   TTN
Gene Name:   titin
Aliases:   CMD1G, CMH9, CMPD4, DKFZp451N061, EOMFC, FLJ26020, FLJ26409, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2J, TMD
RefSeq:   NC_000002.11 NT_005403.17 NG_011618.2
Ensembl:   ENSG00000155657
Entrez:   7273
UniGene:   Hs.134602
Chromosome Mapping:   2q31

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