This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031547; Intron-spanning
The Axin-related protein Axin2 presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway like its rodent homologs mouse conductin/rat axil. In mouse conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon) beta-catenin glycogen synthase kinase 3-beta and conductin which leads to the degradation of beta-catenin. Apparently the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24 a region that shows frequent loss of heterozygosity in breast cancer neuroblastoma and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq Jul 2008]