PrimePCR™ ddPCR™ Expression Probe Assay: F8, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031517; Intron-spanning

 
Fluorophore:   FAM
List Price:    $195.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5056178
Chromosome Location:   X:154221294-154225294question
Amplicon Length:   147

Gene Information

This gene encodes coagulation factor VIII which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which in the presence of Ca+2 and phospholipids converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein isoform a which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein isoform b which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A a common recessive X-linked coagulation disorder. [provided by RefSeq Jul 2008]

Gene Symbol:   F8
Gene Name:   coagulation factor VIII, procoagulant component
Aliases:   AHF, DXS1253E, F8B, F8C, FVIII, HEMA
RefSeq:   NC_000023.10 NG_005114.1 NG_011403.1 NT_167198.1
Ensembl:   ENSG00000185010
Entrez:   2157
UniGene:   Hs.654450
Chromosome Mapping:   Xq28

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