PrimePCR™ ddPCR™ Expression Probe Assay: PIKFYVE, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031484; Intron-spanning

 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
 

Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5056127
Chromosome Location:   2:209163455-209165761question
Amplicon Length:   120

Gene Information

Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions membrane trafficking and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(35)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq May 2010]

Gene Symbol:   PIKFYVE
Gene Name:   phosphoinositide kinase, FYVE finger containing
Aliases:   CFD, FAB1, FLJ37746, KIAA0981, MGC40423, PIP5K, PIP5K3
RefSeq:   NC_000002.11 NT_005403.17 NG_021188.1
Ensembl:   ENSG00000115020
Entrez:   200576
UniGene:   Hs.732047
Chromosome Mapping:   2q34

Number Description Download
10033487 ddPCR™ Mutation Detection Assays Product Insert, Ver C Click to download
10033173 ddPCR™ Copy Number Variation Assays Product Insert, Ver C Click to download
10041702 PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A Click to download
10047489 ddPCR™ Mutation Detection Assays Product Insert, Ver B Click to download
10048730 PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.