PrimePCR™ ddPCR™ Expression Probe Assay: WT1, Human

PrimePCR

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029257; Intron-spanning

 
Fluorophore:   HEX
List Price:    $207.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5052043
Chromosome Location:   11:32417814-32421550question
Amplicon Length:   167

Gene Information

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern with biallelic and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq Oct 2010]

Gene Symbol:   WT1
Gene Name:   Wilms tumor 1
Aliases:   AWT1, GUD, NPHS4, WAGR, WIT-2, WT33
RefSeq:   NC_000011.9 NT_009237.18 NG_009272.1
Ensembl:   ENSG00000184937
Entrez:   7490
UniGene:   Hs.591980
Chromosome Mapping:   11p13

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