PrimePCR™ ddPCR™ Expression Probe Assay: BYSL, Human

PrimePCR

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028150; Intron-spanning

 
Fluorophore:   HEX
List Price:    $201.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5050203
Chromosome Location:   6:41899466-41900157question
Amplicon Length:   132

Gene Information

Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin tastin and the cytokeratins suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10 the levels of trophinin tastin and bystin decreased and then disappeared from placental villi. [provided by RefSeq Jul 2008]

Gene Symbol:   BYSL
Gene Name:   bystin-like
Aliases:   BYSTIN
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112578
Entrez:   705
UniGene:   Hs.106880
Chromosome Mapping:   6p21.1

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